| # | Title | Length |
|---|---|---|
| 1 | Nintendo Logo | 1000 |
| 2 | Title | 87000 |
| 3 | Yoshi's Island | 27000 |
| 4 | Wandering the Plains | 34000 |
| 5 | Vanilla Dome | 48000 |
| 6 | Forest of Illusion | 38000 |
| 7 | Bowser's Castle Emerges | 7000 |
| 8 | Valley of Bowser | 27000 |
| 9 | Star Road | 30000 |
| 10 | Special World | 37000 |
| 11 | Super Mario Bros. Remix | 181000 |
| 12 | Overworld | 87000 |
| 13 | Overworld (Yoshi) | 87000 |
| 14 | Overworld (Hurry) | 77000 |
| 15 | Overworld (Hurry Yoshi) | 77000 |
| 16 | Athletic | 80000 |
| 17 | Athletic (Yoshi) | 80000 |
| 18 | Athletic (Hurry) | 74000 |
| 19 | Athletic (Hurry Yoshi) | 74000 |
| 20 | Underground | 94000 |
| 21 | Underground (Yoshi) | 94000 |
| 22 | Underground (Hurry) | 73000 |
| 23 | Underground (Yoshi Hurry) | 73000 |
| 24 | Swimming | 101000 |
| 25 | Swimming (Yoshi) | 101000 |
| 26 | Swimming (Hurry) | 89000 |
| 27 | Swimming (Hurry Yoshi) | 89000 |
| 28 | Haunted House | 175000 |
| 29 | Haunted House (Hurry) | 115000 |
| 30 | Fortress | 167000 |
| 31 | Fortress (Hurry) | 119000 |
| 32 | P-Switch | 16000 |
| 33 | P-Switch (Cave) | 16000 |
| 34 | P-Switch (Hurry) | 13000 |
| 35 | P-Switch (Cave Hurry) | 13000 |
| 36 | Invincible | 14000 |
| 37 | Invincible (Cave) | 14000 |
| 38 | Invincible (Hurry) | 12000 |
| 39 | Invincible (Cave Hurry) | 12000 |
| 40 | Bonus Game | 69000 |
| 41 | Bonus Game (Hurry) | 65000 |
| 42 | Bonus Game Clear | 4000 |
| 43 | Boss Battle | 102000 |
| 44 | Boss Battle (Hurry) | 86000 |
| 45 | Egg is Rescued | 5000 |
| 46 | World Clear | 13000 |
| 47 | Course Clear | 9000 |
| 48 | Fade Out! | 1000 |
| 49 | Secret Exit! | 3000 |
| 50 | Fortress Clear | 9000 |
| 51 | Player Down | 4000 |
| 52 | Game Over | 7000 |
| 53 | The Evil King Bowser | 98000 |
| 54 | The Evil King Bowser (Intro) | 9000 |
| 55 | Bowser's Last Attack | 80000 |
| 56 | Bowser's Last Attack (Faster) | 79000 |
| 57 | Bowser Zoom | 6000 |
| 58 | Bowser Zoom Out | 3000 |
| 59 | Peach's Item | 2000 |
| 60 | Bowser Defeated | 8000 |
| 61 | Princess Peach is Rescued | 17000 |
| 62 | Staff Roll | 95000 |
| 63 | The Yoshis are Home | 14000 |
| 64 | Cast List | 139000 |
| 65 | Cast List (Looped) | 107000 |
| 66 | Yoshi | 1000 |